Bacterial Transformation Lab Report

Bacteria are microscopic single-celled organisms. Bacteria can transfer these plasmids and therefore genes to one another.

Biology 22 Molecular Laboratory Report 1 Bacterial Transformation 2 Plasmid Isolation 3 Rflp Analysis Results On Bacterial Transformation Analysis Molecular

Algae Cyanobacteria Tianna Francisco The Ubiquity and Dissemination of Microbes Small PCR tube Primers Plasmid DNA from Week 6 Thermal cycler 10X gel loading solution Agarose gel electrophoresis apparatus 1 agarose gel in 1X TAE buffer SYBR Green Micropipettes and tips UV transilluminator.

. This is based on the natural function of a plasmid to transfer genetic information vital to the survival of the bacteria. Put a colony in each tube 5. A greater the length of heat shock results in a higher transformation rate Materials.

Record data Genetic Transformation Reflections Questions 1 What is. Bacterial Transformation Lab Report Title. The author outlines four basic procedures a colonial growth in the Ecoli seen in two large patches each patch holding hundred individual.

In your report use the ampR plasmid. Bacterial Transformation Virtual Lab Summary of Steps 1. Obtain a pGLO Transformation Kit Label the test tubes.

Put the colony into the tube labeled pGLO Put tube holder on. The bacteria transformed with the GFP plasmid will grow on the agar and produce green colonies. Label one tube with your initials and a and the other tube with your initials and a -.

In this experiment plasmids are inserted into a host E. Bacterial Transformation Lab Report Isabella Grisoli Mariza Francis Freda Sciarappa Anissa Hyde Zahrriah Johnson Dr. Use a sterile loop to pick up a single colony of bacteria from your starter plate.

Four plates were set up with agar in them for the bacteria to feed on and grow. Fill all three tubes 2. This was included so you can get more detail about the actual steps involved in bacterial transformation.

Changes were then made to the bacteria. Bacterial transformation lab Objectives. Tabony AP Biology II 1A1B 1 December 2016 Lol 2 5250 Bacterial Transformation Lab Report Analyzing Results Day 1.

Abstract The purpose of this lab was to insert genes that would make E. Conclusion Overall the lab was very successful. 2 LB plates 2 Kanamycin plates 2 Ampicillin plates Plasmids 1 2 and 3.

Transfer 2-4 large colonies using a sterile plastic loop to each microcentrifuge tube and completely resuspend. This would be 53 816 summing to 65 as our transformation efficiency. Their genetic information is encoded in one large chromosome in addition to plasmids which are small circular molecules of DNA Campbell and Reece 2005.

Place tubes into an ice bath 3. You will than detect the home bases in the following lab. For your lab report you will be using the ampR plasmid from the virtual lab.

This work called The Processes of Bacterial Transformation focuses on systematic steps starting with the introduction of the plasmid lux and control plasmid pUC18 into the Escherichia coli Ecoli. Day of lab 1. Bacterial Transformation Lab Report.

This leads to our ultimate goal of the transformation efficiency by dividing the total number of cells growing on the agar plate by the amount of DNA spread on the agar plate. The next process of DNA extraction involved the. Fill all three tubes 2.

Place both tubes on into the ice. Follow the processs in the Transformation Kit-Quick Guide provided in lab. PGLO and -pGLO Put CaCL2 into both of the tubes utilizing a sterile transfer pipet Put tubes on ice next get a single colony of bacteria.

Bacterial Transformation Virtual Lab Summary of Steps 1. Do not transfer any agar. Swirl the rube 6.

Inoculate each tube 4. And so placed in a icebox to decelerate the growing of the bacterium. Get two microcentrifuge tubes which should each contain 200 uL of cold CaCl2 solution.

The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities of it. Ad Order top products for all your research needs. Be careful not to.

In this lab the goal was to transform the bacteria e-coli to glow in the dark or under a black light. All the results came out as they were supposed to. The home bases will be incubated for 24-48 hours.

The procedures that I included are for a face-to-face lab that uses a different plasmid but the steps are the same. The lux operon is an operon that contains a gene for luciferase. Can the length of heat shock during incubation affect plasmid transfer.

Best-in-class lab materials technologies and services from MilliporeSigma. Genetic transformation is an active uptake of free DNA by a bacterial cell and the incorporation of the genetic information. Coli resistant to ampicillin and to glow.

Open the tubes and using a 100-1000 L micropipette with a sterile tip transfer 250 L of the ice cold transformation solution CaCl 2 3. Lab Report 4 Microbial Phototrophs.

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